Vinblastine Poisoning

Photomed Laser Surg.  2011 Apr;29(4):233-7. Epub 2010 Dec 23.

Effect of photobiomodulation on vinblastine-poisoned murine HERS cells.

Hodgson BD, Pyszka B, Henry MM, Buchmann E, Whelan HT.

Source

Marquette University, Milwaukee, Wisconsin, USA. brian.hodgson@marquette.edu

Abstract

OBJECTIVE:

The aim of this study was to investigate the effect of near-infrared (NIR) photobiomodulation on the proliferation and glutathione levels in murine Hertwig’s epithelial root sheath (HERS) cells after poisoning with vinblastine.

BACKGROUND:

Photobiomodulation has been shown to improve wound healing in a number of animal models. There have been no studies on the effect of photobiomodulation on cancer-related chemotherapy injury to the cells that initiate tooth root growth.

MATERIALS AND METHODS:

Control groups consisted of murine HERS cells without vinblastine (VB-) and cells with vinblastine at 10, 20, and 30?ng/mL (VB10, VB20, and VB30). Experimental groups consisted of these same groups with light therapy (VB-L, VB10L, VB20L, and VB30L). The cells were exposed to vinblastine for 1?h. Photobiomodulation consisted of a 75-cm(2) gallium-aluminum-arsenide light-emitting diode (LED) array at an energy density of 12.8?J/cm(2), delivered with 50?mW/cm(2) power over 256?s.

RESULTS:

Vinblastine alone significantly decreased HERS cell proliferation and glutathione levels at all concentrations (VB10 [-55%, p?<?1.0?×?10(-8)]; VB20 [-72%, p?<?1.0?×?10(-9)]; VB30 [-80%, p?<?1.0?×?10(-10)]; and VB10 [-36%, p?<?0.0001]; VB20 [-49%, p?<?1.0?×?10(-6)]; VB30 [-53%, p?<?1.0?×?10(-7)] respectively). Photobiomodulation significantly increased cell proliferation at all levels of vinblastine exposure (VB10L [+50%, p?<?0.0001]; VB20L [+45%, p?<?0.05]; VB30 [+39%, p?<?0.05]) but not of the control (+22%, p?=?0.063). The photobiomodulation significantly increased glutathione production in all concentrations of vinblastine except 20?ng/mL (VB10L [+39%, p?=?0.007]; VB20L [+19%, p?=?0.087]; VB30 [+14%, p?=?0.025]) and the control (+12%, p?=?0.13).

CONCLUSIONS:

Photobiomodulation demonstrated an improvement in proliferation and glutathione levels in vinblastine-poisoned murine HERS cells.