Radioprotective Effect

Adv Gerontol. 2010;23(4):547-53.

Induced thymus aging: radiation model and application perspective for low intensive laser radiation.

[Article in Russian]
Sevost’ianova NN, Trofimov AV, Lin’kova NS, Poliakova VO, Kvetno? IM.

Abstract

The influence of gamma-radiation on morphofunctional state of thymus is rather like as natural thymus aging. However gamma-radiation model of thymus aging widely used to investigate geroprotectors has many shortcomings and limitations. Gamma-radiation can induce irreversible changes in thymus very often. These changes are more intensive in comparison with changes, which can be observed at natural thymus aging. Low intensive laser radiation can not destroy structure of thymus and its effects are rather like as natural thymus aging in comparison with gamma-radiation effects. There are many parameters of low intensive laser radiation, which can be changed to improve morphofunctional thymus characteristics in aging model. Using low intensive laser radiation in thymus aging model can be very perspective for investigations of aging immune system.

Vopr Kurortol Fizioter Lech Fiz Kult. 2010 Jul-Aug;(4):3-6.

The use of drinking sulfate mineral water in combination with laser and magnetic-laser irradiation for primary prophylaxis of post-radiation disorders(experimental study).

[Article in Russian]

Korolev IuN, Mikha?lik LV, Geniatulina MS, Nikulina LA.

Abstract

Experiments on laboratory rats have demonstrated that prophylactic use of drinkable sulfate mineral water in combination with laser and magnetolaser irradiation of adrenal glands produces a radioprotecive effect that was especially well apparent in the liver and less so in the testes. Simultaneously, the functional activity of the thymus decreased. Protection of the liver by the combination of the above factors was more pronounced than the effect of each of them applied separately.

Aviakosm Ekolog Med. 2009 Mar-Apr;43(2):32-6.

Some specific effects of the combined exposure to gamma and laser radiations on survivability of mouse fibroblasts in vitro.

[Article in Russian]

Voskanian KSh, Mitsyn GV, Gaevski? VN.

Abstract

Effects of the combined exposure to gamma-rays and laser radiation (633 nm) on survivability of fibroblast cells C3H/10T1/2, clone 8 were studied. First, a monolayer of fibroblast cells on a plastic vial wall was irradiated by gamma-rays and then the whole wall surface (25 cm2) or the central part of the vial (1 CM2) only was exposed to laser radiation. Forty minutes after exposure the cells were dispersed in 0.25% solution of tripsin and inoculated in vials for survivability determination. The experiments evidenced that the radioprotective effect of laser is essentially invariant no matter the size of a laser-irradiated monolayer. Adding the nutrient medium from the vials subjected to the combined irradiation to the gamma-irradiated vials also increased cell survivability. Cells from the cultivated blend of suspensions (exposed to ionizing and optic radiations and to ionizing radiation only) also exhibited a better survivability. A bystander radioprotective effect of laser can be assumed.

Aviakosm Ekolog Med. 2007 May-Jun;41(3):32-5.

Radioprotective effect of helium-neon laser radiation for fibroblast cells

[Article in Russian]

Voskanian KSh, Mitsyn GV, Gaevski? VN.

Abstract

Effects of combined exposure to 633-nm laser waves and gamma-radiation, and laser waves and protons with the energy of 150 MeV on survivablilty of mice fibroblast cells C3H10T1/2 were compared. Cell suspension (1 – 5 x 10(5) cells/ml) was distributed in 2-ml plastic vials with 1 cm in diameter time interval between two exposures in a combination was no more than 60 s. immediately after exposure a required quantity of cells was inoculated in special vials for survivability assessment. Based on results of the experiment, preliminary and repeated laser treatment was favorable to survivability of fibroblast cells subjected to gamma- or proton irradiation (dose variation factor was within 1.3 to 2.2). Simultaneous exposure of C3H10T1/2 cells to the laser and proton beams also increased their survivability. The radioprotective effect of the helium-neon laser on fibroblasts earlier exposed to ionizing radiation is of chief interest, as most of the present-day radioprotectors are effective only if introduced into organism prior to exposure.

EMLA Laser Health J 2007;2:46-67
European Medical Laser Association (EMLA)

Radioprotective effect of LLLT in whole-body irradiated mice

L. Navratil 1,2, P. Kuna 1, L. Beránek 3, P. Poucková 2, M. Zadinová 2, R. Havránková

1. University of South Bohemia in Ceske Budejovice, Department of Radiology and Toxicology 2. Charles University. 1st Medical Faculty in Prague, Institute of Biophysics and Informatics 3. University of South Bohemia in Ceske Budejovice, Dept. of Computer Science

Radioprotective effect of LLLT administered in dosages of 99 J/cm 2 has been proved in our study. The results are considered partial and stimulating for further experiments.