Protection of Red Blood Cells – Heart Lung Machine

Artif Organs. 2000 Nov;24(11):870-3.

Low power laser protects human erythrocytes In an In vitro model of artificial heart-lung machines.

Itoh T, Murakami H, Orihashi K, Sueda T, Kusumoto Y, Kakehashi M, Matsuura Y. First Department of Surgery Second Department of Anatomy Institute of Health Sciences, Hiroshima University School of Medicine, Hiroshima, Japan.

The protective effect of the low power helium-neon (He-Ne) laser against the damage of human erythrocytes in whole blood was examined in a perfusion model using an artificial heart-lung machine. Preserved human whole blood was diluted and perfused in 2 closed circuits with a double roller pump. The laser irradiated one of the circuits (laser group), and none the other (control group). In the laser group, erythrocyte deformability and erythrocyte adenosine triphosphate (ATP) levels were significantly higher, and free hemoglobin levels were significantly lower than those in the control group. Subsequent morphological findings by means of scanning electron microscope were consistent with these results. Low power He-Ne laser protected human erythrocytes in the preserved diluted whole blood from the damage caused by experimental artificial heart-lung machines. The clinical application of low power laser treatment for extracorporeal circulation is suggested.

Hiroshima J Med Sci. 1996 Mar;45(1):15-22.

The protective effect of low power He-Ne laser against erythrocytic damage caused by artificial heart-lung machines.

Itoh T, Murakami H, Orihashi K, Sueda T, Matsuura Y.

First Department of Surgery, Hiroshima University School of Medicine, Japan.

Abstract

We studied the protective effects of low power He-Ne laser irradiation on erythrocytes against the damage caused by experimental artificial heart-lung machines. The erythrocyte suspension in a closed circuit was perfused with a roller pump. One of two circuits was used for laser treatment and the other for control. The laser group demonstrated significantly higher erythrocyte deformability and erythrocyte ATP levels, and lower free hemoglobin and lipid peroxide levels. A laser output power of 8.5 mW was most effective in protecting the erythrocytes. A further morphological finding using a scanning electron microscope showed fewer echinocytes and more discocytes in the laser group. This finding was consistent with the results of physiological and biochemical tests. Low power He-Ne laser irradiation was shown to protect human erythrocytes from the damage caused by an experimental artificial heart-lung machine, raising the possibility of the clinical application of low power He-Ne laser treatment during extracorporeal circulation in cardiovascular surgery.