Ozone therapy and phototherapy with polarized polychromatic light in treatment of patients suffering from lower limb critical ischaemia.
Chair of Faculty Surgery, Surgut State University of the Khanty-Mansi Autonomous District-Yugra, Surgut, Tyumen Region, Russia.
The authors generalized their experience in treating a total of 77 patients presenting with atherosclerosis of the arteries of lower limbs with degree III-IV ischaemia according to the A.V. Pokrovsky-Fontain classification. The patients were subjected to comprehensive treatment including the impact of piler-light (apparatus Bioptron 2) and ozone therapy. The control group was composed of 66 people receiving conventional therapy. The two groups were comparable by the nosological entity of the disease, gender, age, and the nature of accompanying pathology. Despite carried out classical anticoagulation therapy there was a tendency towards hypercoagulation in phase I (formation of prothrombinase) and phase III (formation of fibrin) of plasma haemostasis, as well as insufficiency of the fibrinolytic system. The obtained results showed direct influence of ozone therapy and piler light on phase I and III plasmatic haemostasis, as well as enhanced fibrinolytic activity of blood on the background of their administration thereof.
The comparative assessment of the wound-healing effects of the treatment with the use of Bioptron, Minitag, Orion+ apparatuses and hollow cathode lamps (experimental study).
The objective of the present experimental study was the comparative assessment of the wound-healing effects of radiation emitted from Bioptron, Minitag, Orion+ apparatuses and hollow cathode lamps (HCL). The emitters of any type were shown to be equally efficacious in that they accelerated wound epithelization by 30% on the average compared with control. Based on the difference between spectral and power characteristics of different sources of radiation and dynamics of their wound-healing efficacy (including that of two types of HCL), the authors arrived at the conclusion that the further development of the proposed approach to wound healing is a promising line of research in the field of spectral phototherapy.
Photomed Laser Surg. 2006 Feb;24(1):17-21.
Effect of linear polarized light irradiation near the stellate ganglion in skin blood flow of fingers in patients with progressive systemic sclerosis.
Lee CH, Chen GS, Yu HS.
Department of Dermatology, Kaohsiung Medical University, Kaohsiung, Taiwan.
OBJECTIVE: The purpose of this study is to evaluate the effect of linear polarized light irradiation near the stellate ganglion area on cutaneous blood flow in fingers of patients with progressive systemic sclerosis. BACKGROUND DATA: Sympathetic overactivity is known to be present in patients with progressive systemic sclerosis. Recently introduced linear polarized light irradiation is designed to simulate noninvasive stellate ganglion block to decrease sympathetic output. METHODS: Five patients with progressive systemic sclerosis and three normal healthy controls were studied. Linear polarized light (Super Lizer) was irradiated near the stellate ganglion on the right side of the neck at 358 J/cm(2) for 10 min. Then, laser Doppler flowmetry, laser Doppler imager, and capillary microscopy were used to measure the cutaneous blood flow of the right fourth finger for 30 min. RESULTS: No significant alternations of the skin blood flow between normal controls and patients with progressive systemic sclerosis after linear polarized light irradiation were detected. The effect of linear polarized light on the microcirculation of patients with progressive systemic sclerosis was minimal and transient. CONCLUSION: The effect of linear polarized light in treating patients with progressive systemic sclerosis may not result from the improvement of skin blood flow. Therefore, the use of linear polarized light in those patients to increase cutaneous blood flow should not be overemphasized.
J Clin Laser Med Surg. 2004 Feb;22(1):59-66.
Effects of low-intensity polarized visible laser radiation on skin burns: a light microscopy study.
Ribeiro MS, Da Silva Dde F, De Araújo CE, De Oliveira SF, Pelegrini CM, Zorn TM, Zezell DM.
Center for Lasers and Applications, IPEN-CNEN/SP, Cidade Universitária, São Paulo, Brazil. email@example.com
OBJECTIVE: This study was carried out to investigate the influence of low-intensity polarized visible laser radiation on the acceleration of skin wound healing. Background Data: Low-level laser therapy (LLLT) at adequate wavelength, intensity, and dose can accelerate tissue repair. However, there is still unclear information about light characteristics, such as coherence and polarization. Some studies indicate that linearly polarized light can survive through long propagation distance in biological tissue. MATERIALS AND METHODS: Three burns about 6 mm in diameter were created on the back of rats with liquid N(2). Lesion “L(//)” was irradiated by He-Ne laser (lambda = 632.8 nm), D= 1.0 J/cm(2), with linear polarization parallel to the spinal column of the rat. Lesion “L(inverted v)” was irradiated using the same laser and dose, but the light polarization was aligned perpendicularly to the relative orientation. Lesion “C” was not irradiated in order to be considered as control. The animals were sacrificed at day 3-17 after lesion creation. Samples were collected and prepared for histological analysis. RESULTS: Histological analysis showed that the healing of irradiated wounds was faster than that of non-irradiated wounds. Moreover, it was observed that skin wound repair is dependent on polarization orientation with respect to a referential axis as the animal’s spinal column. Consequently, “L(//)” was completely healed after 17 days, whereas “L (perpendicular) ” showed a moderate degree of healing after the same period. CONCLUSIONS: These results indicate that the relative direction of the laser polarization plays an important role in the wound healing process when highly coherent He-Ne laser is used.
Masui. 2001 Sep;50(9):958-63.
Effects of low reactive level laser, linear polarized light and Xenon-ray irradiation on the stellate ganglion in dogs
[Article in Japanese]
Nagao M, Yamaguchi S, Okuda Y.
First Department of Anesthesiology, Dokkyo University School of Medicine, Tochigi 321-0293.
The aim of this study was to clarify the influence of low reactive level laser, linear polarized light and Xenon-ray irradiation on stellate ganglion activity in dogs. Under general anesthesia, the right stellate ganglion was exposed by thoracotomy. After stabilization of the hemodynamic parameters, the following baseline measurements were taken: mean arterial pressure (MAP), heart rate (HR), and right brachial artery blood flow (BABF). Experiment 1: The stellate ganglion was directly irradiated for 10 min with low reactive level laser, linear polarized light or Xenon-ray. The hemodynamic parameters were measured for 60 min after each irradiation. Immediately after the irradiation study, stellate ganglion blockade (SGB) with 0.5% mepivacaine 1.0 ml was performed. Experiment 2: After confirming a decrease in BABF by direct electrical stimulation on the stellate ganglion, the stellate ganglion was directly irradiated with low reactive level laser, linear polarized light or Xenon-ray. The hemodynamic parameters were measured for 60 min after each irradiation. Immediately after the irradiation study with 0.5% mepivacaine 1.0 ml was performed. The hemodynamic parameters were also measured for 60 min after SGB. The changes in variables were not statistically significant after each irradiation in the experiment 1 and 2. After SGB, a significant decrease in HR and a significant increase in BABF occurred in the experiment 1 and 2. In conclusion, this study demonstrated that irradiation with low reactive level laser, linear polarized light and Xenon-ray of the stellate ganglion did not induce sympathetic blockade in dogs.
Masui. 1992 Nov;41(11):1814-7.
Polarized light irradiation near the stellate ganglion in a patient with Raynaud’s sign
[Article in Japanese]
Otsuka H, Okubo K, Imai M, Kaseno S, Kemmotsu O.
Department of Anesthesiology, Hokkaido University School of Medicine, Sapporo.
Polarized light irradiation near the stellate ganglion was performed in a 55-year-old female with Raynaud’s sign. She was suffering from cold and numb pain in bilateral fingers for 1 year. Stellate ganglion block and low reactive-level laser therapy near the stellate ganglion were not sufficient to relieve this symptom. Polarized light irradiation near the stellate ganglion induced a sting stimulation and warm sensation in her hands. Thermograms revealed a remarkable increase in temperature of her hands. The results imply that polarized light irradiation near the stellate ganglion increases blood flow of forearms and relieves Raynaud’s sign.
CHANGES IN SOME FUNCTIONAL AND BIOCHEMICAL PARAMETERS OF THE CIRCULATING HUMAN BLOOD AFTER PERCUTANEOUS APPLICATION OF VISIBLE POLARIZED LIGHT AT A THERAPEUTIC DOSE
M.F.Balljuzek, N.Yu.Romanenko, S.I.Morozova, E.A.Manova, 1K.A.Samoilova, 1A.V.Vologdina.
Center of Phototherapy and Laser Surgery of the Russian Academy of Sciences Hospital, 1Institute of Cytology of the Russian Academy of Sciences, St.Petersburg, Russia
Three years ago, our clinics started application of a Swiss phototherapeutic device BIOPTRON that allows an exposure of a small body area to visible incoherent polarized (VIP) light, whose spectrum and power density are close to the Sun visible radiation (400-2000 nm, 95% of polarization, 12 J/cm2). To elucidate physiological mechanisms and to specify indications for this type of phototherapy,we analyzed some biochemcal parameters in 26 healthy volunteers during the course of 10 daily irradiations of a body area, 400 cm2.In the Placebo group (n=16) the irradiation was imitated and 5 exfusions of 140 ml blood for 10 days for study was performed, like in the main group. A rapid statistically significant decrease of the glucose level in 65% of VIP-exposed volunteers (on average, by 10%) has been observed in 0.5 hr after VIP-session but no significant changes in the Placebo group. Subsequently, strongly depending on the initial level, there was a decrease or an increase of this parameter in most VIP-irradiated subjects. The correlation and dispersion analysis has revealed regulatory and normalizing effect of the VIP-sessions on the glucose content. In the Placebo group, this regularity was not detected. Deep changes were in parameters of lipid metabolism: an immediate decrease of triglyceride content, (from 1.14 to 1.00 mmole/l) and its gradual, statistically significant decrease by the end of the course. The analysis indicated both a regulatory and normalizing effect of the VIP-course on the content of these atherogenic lipids The increase in b-lypoproteins’ level was observed in 24 hr as well as by the end of course in both groups. Hence, this effect was due to the hemoexfusion rather than the effect of light.A statistically significant increase of the level of anti-atherogenic lipid a-cholesterol (from 1,76 to 1.98 mmol/l), was found out at all periods of the VIP-course, whereas in the Placebo group this parameter decreased. The data obtained have allowed us to apply the VIP-therapy in 10 patients with X-syndrome. For comparison 10 other patients as well as 12 volunteers were irradiated with therapeutic doses of He-Ne laser light. A small number of examined subjects allows only preliminary conclusions. Unlike volunteers no significant decrease was revealed in the sugar and triglyceride levels, whereas b-lipoproteins’ content decreased soon after the 1st session and by the end of the course of both VIP-therapy and laser treatment (by 32%). As to a-cholesterol, it increased in patients with initially low values and decreased in subjects with high level both after a single exposure to light and by the end of course. There were no significant differences in the studied parameters between the VIP- and laser-treated volunteers.
CHANGES OF THE CONTENT OF SOME CYTOKINES AND GROWTH FACTORS IN CIRCULATING BLOOD OF VOLUNTEERS EXPOSED TO VISIBLE POLARIZED LIGHT AT A THERAPEUTIC DOSE
K.A.Samoilova, D.I.Sokolov, K.D.Obolenskaya, N.A.Zhevago
Institute of Cytology of the Russian Academy of Sciences, St. Petersburg, Russia
The promotion of wound healing and immunomodulation are the cardinal indications for laser therapy. Since these complex phenomena involve participation of immunomodulators and growth factors circulating in blood, it is necessary to study effects of phototherapy their level. Using an ELISA, we determined the plasma levels of Il-b, TNF-a, INF-g, Il-10, and transforming growth factor (TGF-b) in healthy volunteers (n=15-20) in 0, 0.5, and 24 hr after exposures of their back (400 cm2) to a therapeutic dose of visible incoherent polarized (VIP) light (Swiss BIOPTRON-2 device, 400-2000 nm, 95% polarization, 12 J/cm2). As a control (Placebo) group, unexposed people were similarly tested after 2 exfusions of blood for study (30-40 ml for 24 hr). There were no changes of the IL-1b level in 0.5 and 24 hr after the VIP-exposure of 17 volunteers, however, the TNF-a content in 0.5 hr changed in 85% of the subjects: a very slight increase was revealed in persons with a low initial cytokine level (50% of all tested) and a more marked decrease, in volunteers with a high initial cytokine level. The inverse dependence of the light-induced effects on the initial TNF-a levels is confirmed by a negative correlation coefficient r (-0.67). In other 22 volunteers a statistically significant increase in the INF-g level was found in 0.5 and 24 hr: its mean amount changed by 44 pg/ml (from 21 to 65 pg/ml), in some people, by 102-308 pg/ml. Meanwhile, in the Placebo group (n=7) a decrease in the INF-g content was observed, which presumably resulted from the hemoexfusion. There also were changes of the Il-10 and TGF-b levels in 78-85% of volunteers, a high dependence of these changes being found on the initial level: r=-0.96 (Il-10) and r=-0.60 (TGF-b). Indeed, in subjects with the low values of Il-10 (40% of cases) its level increased in 0.5 hr (from 9 to 49 pg/m) and, to a lesser degree, in 24 hr, while in the group with the high Il-10 content it fell markedly (from 150 to 5 pg/ml); in the Placebo group a significant increase of Il-10 was observed (from 67 to 126 pg/ml in 24 h). As to TGF-b, its content within 0.5 hr decreased almost twice in 40% of cases (from 20.4 to 11 ng/ml) and rose in the 40% of volunteers 1.5 times (from 7.9 to 11.8 ng/ml). In the Placebo group there was an elevation of the TGF-b level in 60% of the people, while a decrease, only in 27% of the tested persons (by 25%). However, in 24 hr the 2-fold fall of the TGF-b content was revealed already in 71% of the control group persons, whereas among the irradiated volunteers, it was revealed only in 40%. Thus, a single VIP-exposure of healthy people immediately increases the plasma INF-g level and produces a regulatory effect on the TNF-b, Il-10, and TGF-b content.