Heart Disease

Photomed Laser Surg. 2013 Sep;31(9):447-52. doi: 10.1089/pho.2013.3481.

Effect of lowlevel laser irradiation on oxygen free radicals and ventricular remodeling in the infarcted rat heart.

Yang J1, Huang Z, Zhou Y, Sai S, Zhu F, Lv R, Fa X.

Author information

  • 11 Department of Cardiovascular Surgery, the Second Affiliated Hospital of Zhengzhou University , Zhengzhou, People's Republic of China .

Abstract

OBJECTIVE:

The purpose of this study was to assess the effects of lowlevel laser irradiation (LLLI) on the expression of oxygen free radicals (OFR) and ventricular remodeling (VR) in the model of rat myocardial infarction (RMMI).

BACKGROUND DATA:

LLLI reduces the infarct size and formation of scar tissue in the rat heart after myocardial infarction (MI). However, the exact mechanism has not been demonstrated so far. Methods: RMMI was induced by ligating the left anterior descending coronary artery (LAD). After 3 weeks, LLLI (635 nm, 6 mW laser, 7.64 mW/cm(2), 125 sec, 0.96 J/cm(2)) was applied to the surface of heart directly. Four to six rats were euthanized at 1 h, 24 h, 48 h, 72 h, and 1 week after LLLI, and the infarcted myocardia were excised for the measurement of the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA). At the end of 4 weeks after MI, the hearts were harvested for histological analysis.

RESULTS:

Myocardial SOD activity with LLLI was lower compared with control (p<0.05), and myocardial MDA content with LLLI was higher compared with control (p<0.05), at all the time points. In all rats, the activity of SOD was down to the minimum and the content of MDA was up to the peak at 48 h after laser irradiation. The infarct size was reduced (35±10% vs. 18±9%, p<0.05), the left ventricular wall thickness was increased (0.31±0.03 vs. 0.84±0.02 mm, p<0.05) and the percentage of collagen fibers in the infarcted area was attenuated (64.34±2.20% vs. 30.97±2.60%) by LLLI.

CONCLUSIONS:

LLLI could cause OFR accumulation, reduce infarct size, increase ventricular wall thickness, and attenuate the formation of collagen fibers, suggesting the beneficial effects of LLLI on improvement of VR after MI.

Lasers Med Sci. 2013 Sep;28(5):1305-13. doi: 10.1007/s10103-012-1231-z. Epub 2012 Nov 24.

Infrared lowlevel diode laser on inflammatory process modulation in mice: pro- and anti-inflammatory cytokines.

Fukuda TY1, Tanji MM, Silva SR, Sato MN, Plapler H.

Author information

  • 1Physical Therapy Sector, Irmandade da Santa Casa de Misericórdia de São Paulo (ISCMSP), São Paulo, São Paulo, Brazil. tfukuda10@yahoo.com.br

Abstract

To evaluate the modulation of proinflammatory (interleukin-6, IL-6; tumor necrosis factor-α, TNF-α; and interferon-γ, IFN-γ) and anti-inflammatory cytokines (transforming growth factor-β1, TGF-β1) in the inflammation processes in vivo with lowlevel laser action, 50 isogenic mice were randomly distributed into three groups: control (no surgical procedure, n = 10), sham (surgical procedure with three standard cutaneous incisions, followed by an abdominal muscle incision and suture, n = 20), and laser (same procedure followed by laser exposure, n = 20). The sham group was divided into three subgroups: sham I (euthanasia and evaluation, 36 h after surgical procedure), sham II (euthanasia and evaluation, 60 h after surgical procedure), and sham III (euthanasia and evaluation, 84 h after surgical procedure). The laser group was also divided in three subgroups: laser I (a single lasersession, 12 h after surgery), laser II (two laser sessions, 12 and 36 h after surgery), and laser III (three laser sessions, 12, 36, and 60 h after surgery). All animals in the laser groups received three points per session of continuous infrared laser (wavelength of 780 nm, power of 20 mW, fluency of 10 J/cm(2), exposure time of 20 s per point, and energy of 0.4 J). After euthanasia, spleen mononuclear cells were isolated and cultured for 48 h. Concentrations of IL-6, TNF-α, IFN-γ, and TGF-β1 were obtained by enzyme-linked immunosorbent assay method. There was a significant difference between the IL-6 and TNF-α concentrations in the 60-and 84-h evaluations when the laser and sham groups were compared to the control group (p < 0.05), except for laser II in the TNF-α analysis (p > 0.05). The IFN-γ concentration analysis showed a significant difference only in sham II when compared to the control group (p < 0.05). Thus, there was a modulatory effect of TNF-α and IFN-γ in the laser group, particularly in the 60-h postoperative evaluation. There was no significant difference between the laser, sham, and control groups for TGF-β1 analysis (p > 0.05). The lowlevellaser application decreased the TNF-α and IFN-γ release in vivo of spleen mononuclear cells in mice, especially after two exposure sessions. However, there was no modulation of the IL-6 and TGF-β1 release.

Lasers Med Sci. 2013 May;28(3):1007-16. doi: 10.1007/s10103-012-1190-4. Epub 2012 Aug 31.

Lowlevel laser therapy improves the inflammatory profile of rats with heart failure.

Hentschke VS1, Jaenisch RB, Schmeing LA, Cavinato PR, Xavier LL, Dal Lago P.

Author information

  • 1Laboratório de Fisiologia, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil.

Abstract

Following heart failure (HF), immune activation leads to an imbalance between pro-inflammatory and anti-inflammatory cytokines. Lowlevel lasertherapy (LLLT) has been used as an anti-inflammatory treatment in several disease conditions. However, the effect of LLLT on the skeletal muscle of rats with HF remains unclear. The present report aimed to evaluate the influence of LLLT on the inflammatory profile of rats with HF. The left coronaryartery was ligated to induce HF and a sham operation was performed in the control groups. Male Wistar rats (n=49) were assigned to one of six groups: placebo sham rats (P-Sham; n=8), LLLT at a dose of 3 J/cm(2) sham rats (3 J/cm(2)-Sham; n=8), LLLT at a dose of 21 J/cm(2) sham rats (21 J/cm(2)-Sham; n=8), placebo HF rats (P-HF; n=9), LLLT at a dose of 3 J/cm(2) HF rats (3 J/cm(2)-HF; n=8), and LLLT at a dose of 21 J/cm(2) HF rats (21 J/cm(2)-HF; n=8). Four weeks after myocardial infarction or sham surgery, rats were subjected to LLLT (InGaAlP 660 nm, spot size 0.035 cm(2), output power 20 mW, power density 0.571 W/cm(2), energy density 3 or 21 J/cm(2), exposure time 5.25 s and 36.75 s) on the right gastrocnemius for 10 consecutive days. LLLT reduced plasma IL-6 levels (61.3 %; P<0.01), TNF-α/IL-10 (61.0 %; P<0.01) and IL-6/IL-10 ratios (77.3 %; P<0.001) and increased IL-10 levels (103 %; P<0.05) in the 21 J/cm(2)-HF group. Moreover, LLLT reduced the TNF-α (20.1 % and 21.3 %; both P<0.05) and IL-6 levels (54.3 % and 37.8 %; P<0.01 and P<0.05, respectively) and the IL-6/IL-10 ratio (59.7 % and 42.2 %; P<0.001 and P<0.05, respectively) and increased IL-10 levels (81.0 % and 85.1 %; both P<0.05) and the IL-10/TNF-α ratio (171.5 % and 119.8 %; P<0.001 and P<0.05, respectively) in the gastrocnemius in the 3 J/cm(2)-HF and 21 J/cm(2)-HF groups. LLLT showed systemic and skeletal muscle anti-inflammatory effects in rats with HF.

Lasers Med Sci.  2012 Aug 31. [Epub ahead of print]

Low-level laser therapy improves the inflammatory profile of rats with heart failure.

Hentschke VS, Jaenisch RB, Schmeing LA, Cavinato PR, Xavier LL, Dal Lago P.

Source

Laboratório de Fisiologia, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil.

Abstract

Following heart failure (HF), immune activation leads to an imbalance between pro-inflammatory and anti-inflammatory cytokines. Low-level laser therapy (LLLT) has been used as an anti-inflammatory treatment in several disease conditions. However, the effect of LLLT on the skeletal muscle of rats with HF remains unclear. The present report aimed to evaluate the influence of LLLT on the inflammatory profile of rats with HF. The left coronary artery was ligated to induce HF and a sham operation was performed in the control groups. Male Wistar rats (n=49) were assigned to one of six groups: placebo sham rats (P-Sham; n=8), LLLT at a dose of 3 J/cm(2) sham rats (3 J/cm(2)-Sham; n=8), LLLT at a dose of 21 J/cm(2) sham rats (21 J/cm(2)-Sham; n=8), placebo HF rats (P-HF; n=9), LLLT at a dose of 3 J/cm(2) HF rats (3 J/cm(2)-HF; n=8), and LLLT at a dose of 21 J/cm(2) HF rats (21 J/cm(2)-HF; n=8). Four weeks after myocardial infarction or sham surgery, rats were subjected to LLLT (InGaAlP 660 nm, spot size 0.035 cm(2), output power 20 mW, power density 0.571 W/cm(2), energy density 3 or 21 J/cm(2), exposure time 5.25 s and 36.75 s) on the right gastrocnemius for 10 consecutive days. LLLT reduced plasma IL-6 levels (61.3 %; P<0.01), TNF-/IL-10 (61.0 %; P<0.01) and IL-6/IL-10 ratios (77.3 %; P?<?0.001) and increased IL-10 levels (103 %; P<0.05) in the 21 J/cm(2)-HF group. Moreover, LLLT reduced the TNF-(20.1 % and 21.3 %; both P=<0.05) and IL-6 levels (54.3 % and 37.8 %; P?<0.01 and P<0.05, respectively) and the IL-6/IL-10 ratio (59.7 % and 42.2 %; P<?0.001 and P<0.05, respectively) and increased IL-10 levels (81.0 % and 85.1 %; both P<0.05) and the IL-10/TNF- ratio (171.5 % and 119.8 %; P<0.001 and P<0.05, respectively) in the gastrocnemius in the 3 J/cm(2)-HF and 21 J/cm(2)-HF groups. LLLT showed systemic and skeletal muscle anti-inflammatory effects in rats with HF.

Ann Biomed Eng. 2012 Feb;40(2):516-33. doi: 10.1007/s10439-011-0454-7. Epub 2011 Nov 2.

The nuts and bolts of lowlevel laser (light) therapy.

Chung H1, Dai T, Sharma SK, Huang YY, Carroll JD, Hamblin MR.

Author information

  • 1Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA.

Abstract

Soon after the discovery of lasers in the 1960s it was realized that laser therapy had the potential to improve wound healing and reduce pain, inflammation and swelling. In recent years the field sometimes known as photobiomodulation has broadened to include light-emitting diodes and other light sources, and the range of wavelengths used now includes many in the red and near infrared. The term "low level laser therapy" or LLLT has become widely recognized and implies the existence of the biphasic dose response or the Arndt-Schulz curve. This review will cover the mechanisms of action of LLLT at a cellular and at a tissular level and will summarize the various light sources and principles of dosimetry that are employed in clinical practice. The range of diseases, injuries, and conditions that can be benefited by LLLT will be summarized with an emphasis on those that have reported randomized controlled clinical trials. Serious life-threatening diseases such as stroke, heart attack, spinal cord injury, and traumatic brain injury may soon be amenable to LLLT therapy.

Photomed Laser Surg.  2011 Feb 24. [Epub ahead of print]

Low-Level Laser Irradiation Alters Cardiac Cytokine Expression Following Acute Myocardial Infarction: A Potential Mechanism for Laser Therapy.

Yang Z, Wu Y, Zhang H, Jin P, Wang W, Hou J, Wei Y, Hu S.

1 Department of Surgery, Cardiovascular Institute and Fu Wai Hospital , Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China .

Abstract

Abstract Objectives: Low-level laser irradiation (LLLI) has the potential of exerting cardioprotective effect following myocardial infarction (MI). The authors hypothesized that LLLI could influence the expression of cardiac cytokines and contribute to the reversal of ventricular remodeling. Background: LLLI regulates the expression of cytokines after tissue damage. However, little is known concerning the alteration of the cardiac cytokine expression profile after LLLI.

Methods: MI was created by coronary ligation. The surviving rats were divided randomly into laser and control groups. 33 rats were exposed to a diode laser (635?nm, 5?mW, CW, laser, beam spot size 0.8?cm(2), 6?mW/cm(2), 150?sec, 0.8?J, 1J/cm(2)) as laser group. Another 33 rats received only coronary ligation and served as control group. 28 rats received a thoracotomy without coronary ligation (sham group). One day after laser irradiation, 5 rats from each group were sacrificed and the heart tissues were analyzed by cytokine antibody arrays. Enzyme-linked immunosorbent assay (ELISA) was performed to confirm its reliability. Two weeks after MI, cardiac function and structure were evaluated by echocardiography and histological study. Results: Cytokine antibody array indicated 4 cytokines were significantly changed after laser therapy. ELISA confirmed that granulocyte-macrophage colony stimulating factor and fractalkine were the cytokines involved in the response to therapeutic laser irradiation. However, there was no difference in cytokine release between various groups at 2 weeks after MI. Although LLLI did not improve the damaged heart function, it did reduce the infarct area expansion.

Conclusions: The antibody-based protein array technology was applied for screening the cytokine expression profile following MI, with or without laser irradiation. The expression of multiple cytokines was regulated in the acute phase after LLLI. Our results revealed a potential novel mechanism for applying laser therapy to the treatment of heart disease.

J Cell Mol Med. 2010 Jul;14(7):1975-87. doi: 10.1111/j.1582-4934.2009.00886.x. Epub 2009 Sep 1.

Low level laser irradiation precondition to create friendly milieu of infarcted myocardium and enhance early survival of transplanted bone marrow cells.

Zhang H1, Hou JF, Shen Y, Wang W, Wei YJ, Hu S.

Author information

  • 1Department of Surgery, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

 

 

Abstract

We suggested that lowlevel laser irradiation (LLLI) precondition prior to cell transplantation might remodel the hostile milieu of infarcted myocardium and subsequently enhance early survival and therapeutic potential of implanted bone marrow mesenchymal stem cells (BMSCs). Therefore, in this study we wanted to address: (1) whether LLLI pre-treatment change the local cardiac micro-environment after myocardial infarction (MI) and (2) whether the LLLI preconditions enhance early cell survival and thus improve therapeutic angiogenesis and heart function. MI was induced by left anterior descending artery ligation in female rats. A 635 nm, 5 mW diode laser was performed with energy density of 0.96 J/cm(2) for 150 sec. for the purpose of myocardial precondition. Three weeks later, qualified rats were randomly received with LLLI precondition (n= 26) or without LLLI precondition (n= 27) for LLLI precondition study. Rats that received thoracotomy without coronary ligation were served as sham group (n= 24). In the cell survival study, rats were randomly divided into 4 groups: serum-free culture media injection (n= 8), LLLI precondition and culture media injection (n= 8), 2 million male BMSCs transplantation without LLLI pre-treatment (n= 26) and 2 million male BMSCs transplantation with LLLI precondition (n= 25) group, respectively. Vascular endothelial growth factor (VEGF), glucose-regulated protein 78 (GRP78), superoxide dismutase (SOD) and malondialdehyde (MDA) in the infarcted myocardium were evaluated by Western blotting, real-time PCR and colorimetry, respectively, at 1 hr, 1 day and 1 week after laser irradiation. Cell survival was assayed with quantitative real-time PCR to identify Y chromosome gene and apoptosis was assayed with transferase-mediated dUTP end labelling staining. Capillary density, myogenic differentiation and left ventricular function were tested by immunohistochemistry and echocardiography, respectively, at 1 week. After LLLI precondition, increased VEGF and GRP78 expression, as well as the enhanced SOD activity and inhibited MDA production, was observed. Compared with BMSC transplantation and culture media injection group, although there was no difference in the improved heart function and myogenic differentiation, LLLI precondition significantly enhanced early cell survival rate by 2-fold, decreased the apoptotic percentage of implanted BMSCs in infarcted myocardium and thus increased the number of newly formed capillaries. Taken together, LLLI precondition could be a novel non-invasive approach for intraoperative cell transplantation to enhance cell early survival and therapeutic potential.

 

J Mol Cell Cardiol. 2009 Jan;46(1):4-14. Epub 2008 Sep 30.

Near infrared light protects cardiomyocytes from hypoxia and reoxygenation injury by a nitric oxide dependent mechanism.

Zhang R, Mio Y, Pratt PF, Lohr N, Warltier DC, Whelan HT, Zhu D, Jacobs ER, Medhora M, Bienengraeber M.

Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53326, USA.

Photobiomodulation with near infrared light (NIR) provides cellular protection in various disease models. Previously, infrared light emitted by a low-energy laser has been shown to significantly improve recovery from ischemic injury of the canine heart. The goal of this investigation was to test the hypothesis that NIR (670 nm) from light emitting diodes produces cellular protection against hypoxia and reoxygenation-induced cardiomyocyte injury. Additionally, nitric oxide (NO) was investigated as a potential cellular mediator of NIR. Our results demonstrate that exposure to NIR at the time of reoxygenation protects neonatal rat cardiomyocytes and HL-1 cells from injury, as assessed by lactate dehydrogenase release and MTT assay. Similarly, indices of apoptosis, including caspase 3 activity, annexin binding and the release of cytochrome c from mitochondria into the cytosol, were decreased after NIR treatment. NIR increased NO in cardiomyocytes, and the protective effect of NIR was completely reversed by the NO scavengers carboxy-PTIO and oxyhemoglobin, but only partially blocked by the NO synthase (NOS) inhibitor L-NMMA. Mitochondrial metabolism, measured by ATP synthase activity, was increased by NIR, and NO-induced inhibition of oxygen consumption with substrates for complex I or complex IV was reversed by exposure to NIR. Taken together these data provide evidence for protection against hypoxia and reoxygenation injury in cardiomyocytes by NIR in a manner that is dependent upon NO derived from NOS and non-NOS sources.

Klin Med (Mosk). 2008;86(6):44-7.

Laser Doppler fluometry in assessment of endothelium state in patients with coronary heart disease and its correction by intravenous laser irradiation of blood.

[Article in Russian]

Burduli NM, Gazdanova AA.

Influence of laser therapy on vascular endothelium function in patients with stable angina pectoris, detected by the method of laser Doppler fluorimetry, was studied. 77patients with stable angina pectoris were divided into 2 groups. In control group only medicamentous therapy was used, in main group a course of intravenous blood laser irradiation was carried out additionally. The increase of initially decreased mean index of microcirculation and index of microcirculation after acetylcholine iontophoresis was noticed. Tendency to increase of endothelial oscillations and capillary blood flow under influence of intravenous blood laser irradiation was noticed too. Laser therapy can be considered an effective method of increase of endothelial functional activity in patients with stable angina pectoris.

Kardiol Pol. 2007 Jan;65(1):13-21; discussion 22-3.

Laser biostimulation in end-stage multivessel coronary artery disease – a preliminary observational study.

[Article in English, Polish]

Zyci?ski P, Krzemi?ska-Paku?a M, Peszy?ski-Drews C, Kierus A, Trzos E, Rechci?ski T, Figiel L, Kurpesa M, Plewka M, Chrzanowski L, Drozdz J.

II Katedra i Klinika Kardiologii Uniwersytetu Medycznego, ul. Kniaziewicza 1/5, ?ód?.

BACKGROUND: Low-energy laser radiation through its direct influence on tissue repair processes without heating effect may have vital importance in the therapy of patients with advanced coronary artery disease (CAD). AIM: The introductory assessment of the effects of laser biostimulation applied to patients with advanced multivessel CAD.

METHODS: 39 patients with advanced CAD were assigned (mean age 64.8+/-9.6, male gender 64%, CCS class 2.5+/-0.5, EF=46+/-11%, 69% with a history of acute myocardial infarction), to undergo two sessions of irradiation of low-energy laser light on skin in the chest area from helium-neon B1 lasers. The time of irradiation was 15 minutes while operations were performed 6 days a week for one month. Before including the patients in the experimental group a full clinical evaluation, basic biochemical tests, ECG, 24h Holter recordings, 6-minute walk test, treadmill test using Bruce protocol and full echocardiographic examination were performed. After the first and second period of laser therapy with a one-month break between them analogical parameters with the initial examination were measured.

RESULTS: No side effects associated with the laser biostimulation or performed clinical tests were noted. Lower CCS class (2.5+/-0.5 –> 2.2+/-0.4 –> 2.0+/-0.4, p<0.001), higher exercise capacity (5.1+/-2.2 –> 5.8+/-2.2 –> 6.6+/-2.5 [METS], p=0.023), longer exercise time (257+/-126 –> 286+/-127 –> 325+/-156 [s], p=0.06), less frequent angina symptoms during the treadmill test (65% –> 44% –> 38%, p=0.02), longer distance of 6-minute walk test (341+/-93 –> 405+/-113 –> 450+/-109 [m], p <0.001), lower systolic blood pressure values (SP 130+/-14 –> 125+/-12 –> 124+/-14 [mmHg], p=0.05) and trend towards less frequent 1 mm ST depression lasting 1 min during Holter recordings were noted.

CONCLUSIONS: An improvement of functional capacity and less frequent angina symptoms during exercise tests without a significant change in the left ventricular function were observed. Laser biostimulation in short-term observation was a very safe method. These encouraging results should be confirmed in a larger, placebo-controlled study.

Coron Artery Dis. 2006 May;17(4):385-90.

Long-term results following percutaneous myocardial laser therapy.

Salem M, Rotevatn S, Nordrehaug JE.

Department of Heart Disease, Haukeland University Hospital, Bergen, Norway.

OBJECTIVES: The usefulness and safety of percutaneous myocardial laser therapy in selected patients have been identified in previous 1-year randomized trial reports, including that from a double-blind, sham-controlled trial we independently conducted. We aimed to determine whether the 1-year effects are maintained through a long-term, longitudinal follow-up.

METHODS: Patients (n=77) with chronic, stable, medically refractory angina (class III or IV) not amenable to conventional revascularization and with evidence of reversible ischemia, ejection fraction > or =25%, and myocardial wall thickness > or =8 mm were treated with percutaneous myocardial laser. After the 1-year follow-up and disclosure of all randomized assignments as prespecified in the respective study protocol, patients were followed up longitudinally for a mean of 3 years for angina class, left ventricular ejection fraction, medication usage, and adverse events.

RESULTS: No procedural mortality, myocardial infarction, or cerebral embolism occurred. Pericardiocentesis was required in two patients (2.6%). Cardiac event-free survival was 88% at 1 year and 66% at late follow-up. Mean Canadian Cardiovascular Society angina class was significantly improved from baseline (3.2+/-0.4) at 1 year (2.2+/-1.1, P<0.001) and at a mean of 3 years (1.9+/-1.2, P<0.001). Nitrate usage was significantly reduced at late follow-up; however, ejection fraction did not change over time. In a multivariate analysis, angina improvement at 1 year was found to be a significant independent predictor of both survival and angina improvement at late follow-up.

CONCLUSION: We conclude that percutaneous myocardial laser therapy in selected patients with severe, medically refractory angina not treatable with conventional revascularization induces significant and sustained symptomatic benefit.

Photomed Laser Surg. 2006 Apr;24(2):111-20.

Photoengineering of tissue repair in skeletal and cardiac muscles.

Oron U.

Department of Zoology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Israel. oronu@post.tau.ac.il

This review discusses the application of He-Ne laser irradiation to injured muscles at optimal power densities and optimal timing, which was found to significantly enhance (twofold) muscle regeneration in rats and, even more, in the cold-blooded toads. Multiple and frequent (daily) application of the laser in the toad model was found to be less effective than irradiation on alternate days. It was found that in the ischemia/reperfusion type of injury in the skeletal leg muscles (3 h of ischemia), infrared Ga-Al-As laser irradiation reduced muscle degeneration, increased the cytoprotective heat shock proteins (HSP-70i) content, and produced a twofold increase in total antioxidants. In vitro studies on myogenic satellite cells (SC) revealed that phototherapy restored their proliferation. Phototherapy induced mitogen-activated protein kinase/extracellular signal-regulated protein kinase (MAPK/ERK) phosphorylation in these cells, probably by specific receptor phosphorylation. Cell cycle entry and the accumulation of satellite cells around isolated single myofibers cultured in vitro was also stimulated by phototherapy. Phototherapy also had beneficial effects on mouse, rat, dog and pig ischemic heart models. In these models, it was found that phototherapy markedly and significantly reduced (50-70%) the scar tissue formed after induction of myocardial infarction (MI). The phototherapeutic effect was associated with reduction of ventricular dilatation, preservation of mitochondria and elevation of HSP- 70i and ATP in the infarcted zone. It is concluded that phototherapy using the correct parameters and timing has a markedly beneficial effect on repair processes after injury or ischemia in skeletal and heart muscles. This phenomenon may have clinical applications.

Ter Arkh. 2003;75(12):19-23.

Effect of membrane-stabilizing magnetolaser therapy on cardiodynamics in patients with ischemic heart disease

[Article in Russian]

Vasil’ev AP, Senatorov IuN, Strel’tsova NN, Malishevski? MV, Dubova TV, Zykova EL.

AIM: To evaluate cardiodynamic changes in response to magnetolaser therapy (MLT) and these changes links with lipid shifts in cell membrane.

MATERIAL AND METHODS: The study enrolled 50 patients with effort angina (functional class II-III). Of them, 37 patients were exposed to 10-day courses of MLT, 13 patients were exposed to sham procedures. Before the treatment and 3 months after it measurements were made of lipid peroxidation (LPO) products, structure of erythrocytic membrane and cardiodynamic parameters.

RESULTS: MLT resulted in a significant reduction of LPO products, stabilization of cell membrane structure and positive shifts in cardiodynamics. Correlation was found between the above parameters.

CONCLUSION: Improvement of inotropic, diastolic functions of the myocardium and abatement of cardiac remodeling in coronary heart disease patients in response to MLT is realized primarily due to structural stabilization of cell membrane lipid biolayer.

J Clin Laser Med Surg. 2003 Aug;21(4):231-5.

A preliminary investigation into light-modulated replication of nanobacteria and heart disease.

Sommer AP, Oron U, Pretorius AM, McKay DS, Ciftcioglu N, Mester AR, Kajander EO, Whelan HT.

Central Institute of Biomedical Engineering, University of Ulm, 89081 Ulm, Germany. samoan@gmx.net

OBJECTIVE: The purpose of this preliminary study is to evaluate the effect of various wavelengths of light on nanobacteria (NB).

BACKGROUND DATA: NB and mitochondria use light for biological processes. NB have been described as multifunctional primordial nanovesicles with the potential to utilize solar energy for replication. NB produce slime, a process common to living bacteria. Slime release is an evolutionary important stress-dependent phenomenon increasing the survival chance of individual bacteria in a colony. In the cardiovascular system, stress-induced bacterial colony formation may lead to a deposition of plaque.

METHODS: Cultured NB were irradiated with NASA-LEDs at different wavelengths of light: 670, 728 and 880 nm. Light intensities were about 500k Wm(-2), and energy density was 1 x 10(4) J m(-2).

RESULTS: Monochromatic light clearly affected replication of NB. Maximum replication was achieved at 670 nm.

CONCLUSIONS: The results indicate that suitable wavelengths of light could be instrumental in elevating the vitality level of NB, preventing the production of NB-mediated slime, and simultaneously increasing the vitality level of mitochondria. The finding could stimulate the design of cooperative therapy concepts that could reduce death caused by myocardial infarcts.

Klin Med (Mosk). 2003;81(1):24-7.  

 

Clinico-functional efficacy of medicinal and photon stabilization of cell membrane in patients with angina pectoris

[Article in Russian]

Vasil’ev AP, Senatorov IuN, Strel’tsova NN, Gorbunova TIu.

Modification of erythrocytic membrane and the trend in clinicofunctional indices were studied in 90 patients with angina of effort (FC I-IV) in the course of treatment with a combination of membranoprotective drugs (group 1), magneto-laser radiation (group 2) and imitation of laser radiation (group 3). In patients of groups 1 and 2 the treatment resulted in stabilization of cell membrane accompanied with a hypotensive effect and increased exercise tolerance due to more effective cardiac performance.

Vopr Kurortol Fizioter Lech Fiz Kult. 2003 Jul-Aug;(4):10-3.  

Efficacy of laser therapy in patients with ischemic heart disease

[Article in Russian]

Vasil’ev AP, Strel’tsova NN, Senatorov IuN.

Modification of erythrocytic membrane and the trend in clinicofunctional indices were studied in 93 patients with angina of effort (FC i-IV) in the course of treatment with laser radiation (group 1) and imitation of laser radiation (group 2). In patients of group 1 the treatment resulted in stabilization of cell membrane accompanied with positive cardiodynamic changes.

Vopr Kurortol Fizioter Lech Fiz Kult. 2003 May-Jun;(3):22-5.  

Antioxidant action and therapeutic efficacy of laser irradiation of blood in patients with ischemic heart disease

[Article in Russian]

Volotovskaia AV, Ulashchik VS, Filipovich VN.

Laser irradiation in therapeutic doses (gamma = 632.8 nm, 14 mW) has an antioxidant effect in blood irradiation in vitro as shown by activation of superoxide dismutase (SOD) which is a key enzyme of the antioxidant system (AOS) and suppression of lipid peroxidation. Adjuvant supravascular He-Ne laser irradiation of blood in combined therapy of 82 patients with ischemic heart disease (IHD) produces a positive trend in the clinical picture, hemostasis, lipid metabolism, blood SOD activity. Thereby, this method of laser hemotherapy is recommended for use in IHD patients. The dependence of the treatment results on the initial blood AOS necessitates consideration of AOS state in deciding on laser therapy in this group of patients.

   

Vopr Kurortol Fizioter Lech Fiz Kult. 2002 Jul-Aug;(4):9-11.

Use of infrared laser therapy in patients with ischemic heart disease associated with diabetes mellitus type 2 in health resort

[Article in Russian]

Zin’kovskaia TM, Zavrazhnykh LA, Golubev AD.

Infrared laser therapy (300 Hz) combined with balneotherapy and patients’ education is more effective than standard sanatorium rehabilitation in patients with ischemic heart disease associated with diabetes mellitus type 2. 81.8% patients showed good response manifesting in less frequent anginal attacks, episodes of pain and painless ischemia and lower doses of antianginal drugs. Systolic and diastolic arterial pressure lowered by 18 and 10 mm Hg on the average, respectively. Multimodality rehabilitation of IHD patients with type 2 diabetes mellitus improves hemostasis, carbohydrate and lipid metabolism. Coronary circulation response lasted for 24 weeks

Klin Med (Mosk). 2002;80(4):31-3.  

Diagnostic implications of changed red blood cell count in low-intensity laser radiation of blood in elderly patients with coronary heart disease

[Article in Russian]

Simonenko VB, Siuch NI, Vokuev IA.

Intravenous laser therapy in combination with medication was conducted in 41 elderly patients with coronary heart disease (633 nm, 1 mW, 124 mW/cm2). The study of qualitative and quantitative (osmotic resistance) erythrocyte indices of blood demonstrated the change of erythrocyte number in circulating blood by the third laser procedure. Frequency of these changes correlated with duration of the treatment course. Intravenous laser therapy had a wider spectrum of effects on erythrocyte number than medication. Changes in erythrocyte number in the peripheral blood upon intravenous laser radiation reflects efficiency of treatment of coronary heart disease patients.

Vopr Kurortol Fizioter Lech Fiz Kult. 2001 Nov-Dec;(6):10-3.  

Laser irradiation in the treatment of ischemic heart disease

[Article in Russian]

Vasil’ev AP, Strel’tsova NN, Senatorov IuN.

Cardiodynamic changes due to beta-blocker carvedilol and low-intensity infrared laser radiation were compared in 115 patients with ischemic heart disease (IHD). The comparison has shown a similar positive effect on heart contractility and diastolic function. This gave arguments for feasibility of laser beam usage as a neurohormonal modulator in IHD patients to reduce cardiac remodulation and prevent cardiac failure.

Likars’ka sprava. 2001; (5-6): 111-114.

Evaluation of quantum therapy for the treament of stable angina

[Otsinka efektyvnosti vplyvu kvantovoi terapii na perebig stabil’noi stenokardii].

A total of 68 patients were examined. Of these, 21 had functional class (FC) I stabile angina, 23 presented with FC II angina, 24 had FC III angina. Instituted in the control group patients (n = 30) was standard antianginal therapy (SAT). Laser therapy against the background of SAT employed has been found to improve the functional state of the myocardium, enhance tolerance to physical loads, improve indices for intracardiac hemodynamics. HeNe laser irradiation has an analgecizing effect. Patients with FC I-III exertional angina can derive benefit from laser therapy due to its cardioprotective effect. Positive hemodynamic shifts were accompanied by improvement in general health of patients manifested by lower frequency of angina attacks and episodes of pain-free ischemia of the myocardium. Laser therapy had an effect on relation between painful and painless ischemia of the myocardium as evidenced by a predominant decrease in pain-free episodes of myocardial ischemia, this being regarded as a prognostically favourable fact.

Vopr Kurortol Fizioter Lech Fiz Kult. 2001 Jul-Aug;(4):3-6.  

The efficiency of low-intensity laser radiation in the treatment of arterial hypertension complicated by ischemic heart disease

[Article in Russian]

Shuvalova IN, Klimenko IT, Svinina NG, Tsereteli MV, Zankina VG, Miasoed FR.

The efficiency of low-intensity laser radiation (LILR) was studied in the treatment of 291 patients with arterial hypertension and ischemic heart disease. Clinical grounds are given for use of LILR red and infrared rays in rehabilitation of hypertensive patients with ischemia. The rehabilitation regimens can be differentiated according to the disease severity, type of hemodynamics, state of cerebral circulation.

Vopr Kurortol Fizioter Lech Fiz Kult. 1997 Sep-Oct;(5):9-11.  

The dynamics of the clinico-functional indices in patients with ischemic heart disease under the influence of repeated courses of laser therapy

[Article in Russian]

Vasil’ev AP, Strel’tsova NN.

Repeated courses of laser therapy given to patients with ischemic heart disease, angina of effort class I-IV for 2 years brought about stabilization of coronary insufficiency and improvement of clinical and functional conditions. Microcirculatory picture of the bulbar conjunctiva, coronary reserve improved. The treatment had also a hypotensive effect.

Vopr Kurortol Fizioter Lech Fiz Kult. 1996 Mar-Apr;(2):5-8.

The effect of exposure to magnetics and lasers on the clinical status and the electrophysiological indices of the heart in patients with cardiac arrhythmias

[Article in Russian]

Budnar’ LN, Antiuf’ev VF, Oranskii IE, Bekhter TV.

Magnetolaser radiation has a considerable influence on electrophysiological condition of the sinus node and sinoatrial zone. There are cases when patients with sick sinus syndrome get rid of arrhythmia. The treatment is safe and promising for further studies.

INTRAVENOUSL LASER IRRADIATION OF BLOOD IN COMPLEX REHABILITATION OF THE PATIENTS WITH ISCHEMIC HEART DISEASE IN THE SENIOR AGE GROUPS

Y.M. Kazakov, L.A. Zvyaginceva, et al. Ukrainian medical stomatological academy, Poltava, Ukraine

The problem of rehabilitation of the ischemic heart disease (IHD) patients remains essential. Use of physical methods of treatment of the given category of the patients in connection with numerous lacks in drug treatment (collateral action, decrease of tolerance with long treatment, sometimes their inaccessibility for continuous treatment) gets the increasing distribution. 32 IHD patients are surveyed, from them 6 IHD patients had a combination with arterial hypertension. Average age explore patients – 66±4 years. On a background of a dietary nutrition and basic treatment with nitrates have carried out an irradiation of blood by the helium-neon laser through light-guide in cubital vein with capacity 1,5 mW, exposition – 30 minutes once per day within ten days. As a result of complex treatment there has come an improvement of a common condition of the patients was noted, normalization of rheological parameters of blood. Thus, intravenous laser blood irradiation can be used for the treatment of patients with IHD in stage of the ambulatory rehabilitation, including the patients of the senior age groups.

THE APPLICATION OF LOW-INTENSITY LASER RADIATION FOR THE TREATMENT OF INFARCTIONAL PATIENTS WITH EARLY ANGINA PECTORIS REFRACTERING TO THE DRUG THERAPY

Y.L.Gabinsky, Y.R. Yakovlev, S.V. Yakovleva

Yekaterinburg Infarction Centre, Yekaterinburg, Russia

The results of treatment by low-intensity laser radiation of blood are given with taking into account of individual sensitivity in process of laser therapy. 600 patients with myocardial infarction and early angina pectoris (500 patients simultaneously with drug therapy were given laser therapy, 100 patients were given only drug therapy) were examined. The selecting of dose of laser radiation was based on refraction index of blood plasma in the field of laser radiation in vitro. Before the starting of laser therapy, in the middle of the course and at the end of it the state of health was estimated according to the theory of stress by Selie. In the group of patients, receiving laser therapy, the pronounced positive clinical effect has been registered. According to our data this effect reflects the changing of blood coagulation.

EFFICIENCY OF INTRAVASCULAR BLOOD IRRADIATION WITH HE-NE LASER IN PATIENTS WITH STABLE EFFORT ANGINA PECTORIS DEPENDING ON FUNCTIONAL CLASS

A.I. Koryakov

Sverdlovsk Regional Clinical Psychoneurological Hospital of the Disabled Soldiers, Yekaterinburg, Russia

The aim of this study was the comparison of antianginal effect of intravascular He-Ne laser irradiation of blood (ILIB) in patients with different functional classes of angina pectoris. The tolerance to exercise was studied in 44 men with stable angina, functional classes I-II have been registered in 20 patients, functional classes III-IV – in 24 patients. Four veloergometer tests both before and after the course of invasive treatment were performed for every patient under circumstances of total abolition drugs. All 44 patients were randomized by the blind method into ILIB and placebo groups. Nine men with the slight angina pectoris and 15 patients with the painful angina pectoris were subjected to 6 ILIB seances, the total course dose was 21,6±0.8 J. We used the He-Ne laser radiation (wavelength 632,8 nm) from the three-wave laser therapy device “Adept”, the light power in the end of intravenous light-guide was from I to 2 mW. Other 11 patients with angina functional classes I-II and 9 patients with angina pectoris functional classes III-IV received the placebo course of six invasive procedures. The effect of ILIB was calculated as a difference of increases in the mean-sample maximum workload between the laser therapy group and the placebo group. In patients with severe angina ILIB increased the exercise power reached on veloergometer on the average by 7,3±3,2 W (t = 5,26; p = 0). In contrast, in patients with the high tolerance to exercise ILIB did not significantly affect this parameter. We obtained the great difference in effects of ILIB in patients with the serious angina pectoris and the mild stenocardia (t = 2,41, p = 0,03), Thus, efficacy of the invasive blood irradiation with He-Ne laser in patients with stable effort angina pectoris depended on the initial tolerance to exercise.

INFLUENCE OF INTRAVENOUS BLOOD IRRADIATION WITH HE-NE LASER ON THE ANTIANGINAL EFFECT OF NITROGLYCERIN IN PATIENTS WITH STABLE EFFORT ANGINA PECTORIS

A.I. Koryakov, N.N. Koryakova, A.I. Iofin

Sverdlovsk Regional Clinical Psychoneurological Hospital of the Disabled Soldiers, Yekaterinburg, Russia

We reported about the significant antianginal effect of intravenous He-Ne laser irradiation of blood (ILIB) in patients with serious stable effort angina functional classes 3-4. The goal of this work was to investigate an action of ILIB on an increase of the tolerance to exercise after the sublingual taking nitroglycerin in dose 0.5 mg in patients with stenocardia. We observed 27 men with stable effort angina functional classes II-IV. All patients were subjected two conjugate loading tests on veloergometer with nitroglycerin both before and after of the seven-day treatment. The antianginal effect of nitroglycerin was calculated as the magnitude of the increase maximum workload after taking nitroglycerin in comparison with the initial level of the maximum exercise power. Patients were divided randomly into main and control groups (17 and 10 men accordingly). Patients from the master group were subjected to six seances of ILIB. Power at the light-guide end was 1-2 mW, the accumulated dose was 21.6±0.8 J. The control group received the course of 6 invasive 45-minute placebo procedures that outwardly were like seances of ILIB. Both the intravascular laser treatment and its imitation were performed with three-wave laser therapy device “Adept”. In all patients invasive procedures were combined with antianginal medication treatment that was abolished 1 -2 days before loading tests. Although after the treatment the increase of antianginal effect of nitroglycerin was not statistically significant in both groups, nevertheless after ILIB the rise of nitroglycerin’s effect was more by 63 per cent as compared with placebo (p> 0.9). Thus, ILIB not only increases tolerance to exercise in patients with severe stable effort angina, but also it is not decreases the antianginal effect of nitroglycerin.

Vopr Kurortol Fizioter Lech Fiz Kult. 2002 Jul-Aug;(4):9-11.  

Use of infrared laser therapy in patients with ischemic heart disease associated with diabetes mellitus type 2 in health resort

[Article in Russian]

Zin’kovskaia TM, Zavrazhnykh LA, Golubev AD.

Infrared laser therapy (300 Hz) combined with balneotherapy and patients’ education is more effective than standard sanatorium rehabilitation in patients with ischemic heart disease associated with diabetes mellitus type 2. 81.8% patients showed good response manifesting in less frequent anginal attacks, episodes of pain and painless ischemia and lower doses of antianginal drugs. Systolic and diastolic arterial pressure lowered by 18 and 10 mm Hg on the average, respectively. Multimodality rehabilitation of IHD patients with type 2 diabetes mellitus improves hemostasis, carbohydrate and lipid metabolism. Coronary circulation response lasted for 24 weeks.

Sov Med. 1990;(3):12-5.  

Helium-neon laser therapy in the combined treatment of unstable stenocardia

[Article in Russian]

Korochkin IM, Kapustina GM, Babenko EV, Zhuravleva NIu.

He-Ne laser therapy included in complex of therapeutic methods for patients with unstable angina pectoris is a highly effective treatment modality; it helps essentially reduce the risk of acute myocardial infarction in these patients. Clinical efficacy of laser therapy is confirmed by its favorable action on hemostasis plasma factors, consisting in reduction of fibrinogen level, normalization of antithrombin-III (AT-III), decrease of the level of soluble fibrinomonomer complexes, this indicating a lowering of the blood coagulation potential. Absence of significant changes in plasminogen level may be an indicator of the nonenzymic route of fibrinogen system activation. Sessions of intravenous laser therapy should be administered 2-3 times a week to unstable angina pectoris patients with low AT-III levels, whereas for patients with initially high or normal AT-III levels combined laser therapy is advisable (4-5 daily invasive procedures and 6-8 skin surface ones on the Zakharyin-Head’s zones). Measurements of endogenic anticoagulants is an effective means for monitoring laser therapy in this patient population.

Vestn Khir Im I I Grek. 2000;159(2):60-4.  

The effect of different methods of photohemotherapy on the rheological properties of the blood in patients with ischemic heart disease

[Article in Russian]

Gavrisheva IA, Dutkevich IG, Pleshakov VT, Kolesnik VS.

The authors made an analysis of results of examination of 41 patients with ischemic heart disease treated by the standard medicamentous therapy and when using different methods of photohemotherapy against its background. It was established that medicamentous therapy during 2 weeks failed to result in a substantial improvement of rheological properties of blood, while its combination with photohemotherapy could give a considerable positive effect coinciding with clinical improvement of the patient’s state. Shorter terms are required to correct hemorheological indices when autotransfusions of photomodified blood are used.

Vopr Kurortol Fizioter Lech Fiz Kult. 1995 Jan-Feb;(1):5-7.  

 

The effect of different types of laser therapy on the reactivity of the peripheral blood neutrophils in patients with ischemic heart disease

[Article in Russian]

Siuch NI, Illarionov VE.

The responses to laser therapy (intravenous, continuous skin exposure without a magnet, magnetic laser therapy) of 83 patients with coronary heart disease aged 50-80 demonstrated the advantages of noninvasive laser irradiation of blood. Myeloperoxidase activity may serve a criterion for estimating the number of irradiation procedures needed.

Kardiologiia. 1993;33(2):22-3.  

 

Changes in central hemodynamics and microcirculation during laser therapy in patients with coronary insufficiency

[Article in Russian]

Gel’fgat EB, Samedov RI, Kurbanova ZN, Gadzhiev GG.

The study was undertaken to examine 45 patients with Stages IIB-III heart failure (HF) by the classification developed by V. Kh. Vasilenko and N. D. Strazhesko. Thirty patients had laser therapy in addition to the routine treatment, 15 patients served as a control group. The combined drug treatment along with laser therapy in patients substantially improved peripheral circulatory parameters than in the controls. There was a positive dynamics of central hemodynamic parameters as shown by lower left ventricular volumes and higher myocardial contractile and pump functions. Improvement of microcirculatory and central hemodynamic parameters in patients treated with laser occurred in earlier periods of hospital stay than in the controls.

Vopr Kurortol Fizioter Lech Fiz Kult. 1996 Mar-Apr;(2):3-5. \

 

The laser therapy in patients with hypertension in combination with coronary insufficiency

[Article in Russian]

Kniazeva TA, Badtieva VA, Zubkova SM.

Hypertensive patients with coronary insufficiency have received infrared (lambda = 0.85 microns) laser radiation to the skin. The treatment is shown to have antianginal, antihypertensive effects, to improve cardiac performance, myocardial contractility, to increase myocardial, coronary and aerobic reserves. This clinicofunctional efficacy is accompanied by positive shifts in lipid metabolism, lipid peroxidation activity, antioxidant defense, hemocoagulation and microcirculation.

INFLUENCE OF LOW DOSE LASER THERAPY ON ENDOTHELIAL FUNCTION IN PATIENTS WITH CAD

S. Belousov, E. Galperin, E. Smetova 

The purpose of this study was the evaluation of arterial relaxation ability in 15 patients with CAD before and after the course of infrared laser therapy. 18 controls (healthy men). With high-resolution ultrasound and impulse wave doppler we measured the increasing of volumic velocity flow (%) of the arteria poplitea at rest and during reactive hiperaemia (with increased flow causing endothelium-dependent dilatation). In controls flow-mediated mean dilatation was normal. Endothelial dysfunction is present in majority of patients with CAD, flow-mediated dilatation was much reduced of absent. Course of infrared laser therapy was benefit to restore endothelial function in patients with CAD.

EFFECTS OF LASER THERAPY ON VENTRICULAR AND SUPRAVENTRICULAR EXTRASYSTOLES IN PATIENS WITH ANGINA PECTORIS OF 1-3 FUNCTIONAL CLASSES

V.A. Lyusov, K.G. Khutchumova, Y.N. Fedullaev

The Russian Medical State University, Moscow, Russia

The aim of the present investigation is to study the effect of low level laser on extrasystoles. The amount of citrasystoles is registered before treatment and on the 10th day after the therapy. 64 patients with angina pectoris underwent laser therapy. We registered patients with more than 200 supraventricular extrasystoles and with more than 100 ventricular extrasystoles before our therapy. The treatment was carried out the keep of GaAs laser – “Elat” (890 nm) with pulse regime. The region of projection of carotide sinuses and heart were irradiated with pulse repetition rate 150 – 300 Hz with expositions 20 min. The amount of extrasystoles was counted by Holter monitor device. Laser therapy lessened supraventricular citrasystoles from 308,4±29,6 to 134,6±21,8 (p<0,001) and ventricular extrasystoles – from 182,1±31,3 to 41,5±16,3 (p<0,05) in patients with 1-3 functional class. We observed a decreased number of extrasystoles in 2 FC by 39,9 percent and by 13,7 percent, respectively. On patients with 3 FC – by 38,7 percent (p<0,001) and by 13,3 percent (p<0,05), respectively. This effect could still be observed after two months after the treatment. The results showed, that laser therapy lessened extrasystoles and that it can be applied as an effective means when treating ventricular and supraventricular extrasystoles in patients with angina pectoris 1-3 FC.

THE EFFECTIVENESS AND INFLUENCE ON RHEOLOGICAL BLOOD FEATURES AND ENDOTHELIAN VESSELS FUNCTION OF INFRARED LASER THERAPY IN PATIENTS WITH ANGINA PECTORIS

T.M. Zenkovskaya, O.A. Matronchick, F.A. Khairullina, A.D. Kats

Hospital of War Invalids, Perm State Medical Academy, Russia

One can observe the impairment of endothelian vessels function (EVF) and rheological blood features (RhBF) in patients with various forms of ischemic heart disease. The task of this investigation is the study of infrared laser therapy (IRLT) influence to RhBF, EVF; the dependence of treatment effectiveness from above stated functions. 58 patients with stable angina pectoris III and IV functional class where under supervision. IRLT was performed by means of USOR and ASOR-2K apparatus with rate 300 Hz, the procedure lasted 10 minutes and it was given 10 times. To evaluate treatment effectiveness and its dependence on RhBF we have studied erythrocyte aggregation (EA), we have counted erythrocyte aggregation coefficient (EAC), the erythrocyte flowing (HF), it was marked by the index of flowing (IHF), spontaneous thrombocytcs aggregation (SThA) before and after IRLT and capillary fragility test (CFT). Before IRLT EAC was 0.78±0.04, after it was 0.89±0.06 (p<0.05), standard – 1.0. Before IRLT 1EF was 80,44±0.96%, after it was 90.47±0.91% (p<0.05). SThA was higher standard in the majority of patients before therapy. It decreased after the therapy. After CFT SThA reduced in patients given IRLT (25.81±1.50%, 20.39±0.91% and 17.51±0.11%). The lowering of SThA did not occur in patients treated only with medical preparations. The IRLT was effective in 91.7%. The effect of therapy was higher in patients with reliable lowering of EA, SThA, increased IEF. Therefore the IRLT is highly effective method of angina pectoris treatment and improves rheological properties of blood and endothelium vessels function.

RESULTS OF 10-YEAR USE OF LOW INTENSITY LASER THERAPY AND CONVENTIONAL TREATMENT OF PATIENTS WITH STENOCARDIA

G.W. Babushkina, I.M. Korochkin, A.V. Kartelishev

Bashkir Medical University, Ufa, Russian State Medical University, Moscow, RF

The improvement of treatment and rehabilitation of patients with CHD remains to be a vital problem in cardiology, as morbidity, disability and mortality rates keep steadily growing. However, insufficient efficacy of therapeutic methods, the development of retraction to antianginal drugs, their intolerance make medical men search for the new methods of CHD treatment, nonmedicamental ones inclusive. This paper presents the results of 10-year observation over two groups of patients with CHD (the main and control). 280 patients, who underwent a course of laser therapy (LT), have been included in the main group (A), the control (B) 155 patients have been treated conventionally. Prospective observation demonstrated new cases of acute myocardial infarction (AMI), mortality from CHD as well. There were 26 cases of AMI (II, 9 cases per 1000 population a year) in the main group, 27 AMI cases (27.4 per 1000 people a year) in the control over the observation period. The analysis of the acute attack and AMI development incidence in II – IV functional class (FC) stenocardia patients for 5 years before and after LT annual course demonstrated that the patients who underwent the repeated antirelapsing course of low intensity LT during remission showed reliable advantage (p<0,01 to p<0,05) of the method used as well as its prophylactic effect to prevent CHD progressing which was confirmed by biochemical blood analysis (lipid metabolism and rheologic properties). During the observation period there were 54 deaths: 8,9% in the main and 18,7% in the control group. CHD was the main cause of death in both groups which made 84% and 89.6% respectively of all death causes. Mortality rate was 17,4 and 32,6 respectively per 1000 population a year (p<0,01). On the whole, prospective analysis of the obtained data showed that LT course is directly dependent on its type and frequency of repeated courses and enables to prolong therapeutic remission of CHD by 2,5 times on the average. Thus, low intensity LT is a method of choice for patients suffering from different forms of angina pectoris and is carried out either in combination with conventional drug therapy or alone.