Fertility

Arch Ital Urol Androl. 2015 Mar 31;87(1):14-9. doi: 10.4081/aiua.2015.1.14.

Light-emitting diode exposure enhances sperm motility in men with and without asthenospermia: preliminary results.

Salama N1, El-Sawy M.

Author information

  • 1Department of Urology, Alexandria Faculty of Medicine, Alexandria. nadersalama58@yahoo.com.

Abstract

OBJECTIVE:

To evaluate the effect of lightemitting diode (LED) on sperm motility in men with and without asthenospermia.

MATERIAL AND METHODS:

Semen samples from 27 men were assessed and washed. An aliquot was taken from each sample as a control. The remaining amount was exposed to red LED for 2, 5 and 10 minutes. Sperm motility from the test and control tubes were re-checked at the end of each time interval. In 11 of these 27 samples, the same protocol was repeated without sperm washing. Evaluation of sperm creatine kinase (CK) activity, hypoosmotic swelling (HOS) test and aniline blue staining (ANBS) were undertaken after phototherapy in additional 15 samples.

RESULTS:

Progressive sperm motility increased significantly after LED treatment at the different time intervals whether in washed (p = 0.000) or non-washed (p = 0.003) samples. The amount of the increase in motility in washed aliquots was significantly more (p = 0.000) than in naive semen. Sperm CK activity increased, but was not significant whilst there were no changes regarding HOS and ANBS.

CONCLUSION:

Red LED is a promising safe tool to boost sperm motility in vitro. This may have a great implication on maximizing the possibilities and outcomes of intrauterine insemination trials.

Lasers Med Sci. 2014 Jan;29(1):97-104. doi: 10.1007/s10103-013-1276-7. Epub 2013 Feb 14.

Effect of 830-nm diode laser irradiation on human sperm motility.

Salman Yazdi R1, Bakhshi S, Jannat Alipoor F, Akhoond MR, Borhani S, Farrahi F, Lotfi Panah M, Sadighi Gilani MA.

Author information

  • 1Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran, r_salman_yazdi@yahoo.com.

Abstract

Sperm motility is known as an effective parameter in male fertility, and it depends on energy consumption. Low-level laser irradiation could increase energy supply to the cell by producing adenosine triphosphate. The purpose of this study is to evaluate how the low-level laser irradiation affects the human sperm motility. Fresh human semen specimens of asthenospermic patients were divided into four equal portions and irradiated by 830-nm GaAlAs laser irradiation with varying doses as: 0 (control), 4, 6 and 10 J/cm(2). At the times of 0, 30, 45 and 60 min following irradiation, sperm motilities are assessed by means of computer-aided sperm analysis in all samples. Two additional tests [HOS and sperm chromatin dispersion (SCD) tests] were also performed on the control and high irradiated groups as well. Sperm motility of the control groups significantly decreased after 30, 45 and 60 min of irradiation, while those of irradiated groups remained constant or slightly increased by passing of time. Significant increases have been observed in doses of 4 and 6 J/cm(2) at the times of 60 and 45 min, respectively. SCD test also revealed a non-significant difference. Our results showed that irradiating human sperms with low-level 830-nm diode laser can improve their progressive motility depending on both laser density and post-exposure time.

Laser Ther. 2012 Dec 26;21(4):275-85. doi: 10.5978/islsm.12-OR-16.

The Proximal Priority Theory: An Updated Technique in Low Level Laser Therapy with an 830 nm GaAlAs Laser.

Ohshiro T.

Author information

  • Japan Medical Laser Laboratory, Shinanomachi, Shinjuku, Tokyo, Japan.

 

Abstract

BACKGROUND AND AIMS:

The 830 nm GaAlAs diode laser has played an extremely active role in low level laser therapy (LLLT) since the early 1980's. Recently, the author modified his original proximal priority laser technique (PPLT), and the current article set out to explain the improved approach and show scientific evidence for its efficacy. Laser Therapy System: The laser therapy system used was based on the GaAlAs diode (OhLase-3D1, JMLL, Japan), delivering 60 mW in continuous wave at a wavelength of 830 nm in the near infrared with a power density at the tip of the probe head of approximately 1.2 W/cm(2). Proximal Priority Laser Technique: Under the author's PPLT concept, the brain is the control center for the body so every other part of the body is distal to the head. The main blood supply to the head is through the carotid arteries, and the deep penetration of the 830 nm beam applied to the side of the neck can involve and photoactivate the external and internal carotids, increasing the blood supply to the brain and creating a systemic parasympathetic system-mediated whole-body effect. The author has added gentle neck-stretching, trunk-stretching and his distal tissue softening approaches concomitant with the irradiation which enhance treatment efficacy.

RESULTS:

Real-time fine-plate thermography has revealed whole-body warming as a result of the PPLT, with applications including chronic pain attenuation, female infertility and functional training of paraplegic cerebral palsy patients. The warming effect had a latency from hours to days, increasing in intensity and latency with subsequent PPLT sessions. Both Doppler flowmetry and SPECT have shown increased cerebral and systemic blood flow following PPLT.

CONCLUSIONS:

PPLT is easy to deliver and offers tangible results in a large range of conditions, enhancing the efficacy of diode laser LLLT.

Rev Med Chir Soc Med Nat Iasi. 2012 Oct-Dec;116(4):1131-5.

Noninvasive laser therapy for outpatients with chronic inflammatory disorders of cervix.

Botez M1, Anton C, Mircea R, Anton E.

Author information

  • 1Medical-Center Themis Art, Iasi., University of Medicine and Pharmacy Grigore T Popa Iasi, Faculty of Medicne.

 

Abstract

Chronic inflammation of the cervix can develop cervical stenosis with infertility and cervical congestion is related with the cervical cancer. We create a review of main etiological agents and methods of screening and diagnosis. We also make a brief review of modern therapeutic approach.

CONCLUSIONS:

We follow the utility of LLLT through the following aspects: evolution, indications, results of Babe?-Papanicolau screening, cytology, clinical aspects. The results of the study will allow the complex system of treatment to be used in a large category of women. We appreciate that the procedure (used in our center also) will decrease the cervical pathology, the morbidity inside the treatment, the mortality through the evolution of cervical cancer. We propose the applicability for outpatients first and then as an integrated treatment method inside hospitals for a wide access.

Laser Ther. 2012 Jul 3;21(2):97-103. doi: 10.5978/islsm.12-OR-05.

Personal Overview of the Application of LLLT in Severely Infertile Japanese Females.

Ohshiro T.

Author information

  • Ohshiro Clinic; Japan Medical Laser Laboratory; and Department of Plastic & Reconstructive Surgery, Keio University School of Medicine, Shinanomachi, Tokyo, Japan.

Abstract

BACKGROUND AND AIMS:

The rapidly graying population in Japan is being compounded by the rapidly-dropping birth rate. The latter is mostly due to the later ages at which women are giving birth as the marriage age has also been increasing. Giving birth at a later stage is associated with problems for both mother and child, but for older would-be mothers the greatest problem is infertility, sometimes severe. The present article will show how the application of low level laser therapy (LLLT) is a potentially effective treatment for severe infertility.

SUBJECTS AND METHODS:

Seventy-four females (average age 39.28 yr) with severe infertility in whom assisted reproductive technology (ART) had been unsuccessful (average of 9.13 yr) participated in the first part of a study from October 1996 – April 2000. LLLT was applied (830 nm, CW, GaAlAs 60 mW diode LLLT) in Ohshiro's proximal priority technique (average 21.08 sessions) with or without other ART approaches. Based on successful outcomes, the study was then extended to March 2012, amassing a final total of 701 patients.

RESULTS:

Pregnancy was achieved in the first part of the trial in 16 patients (21.7% of 74) of whom 11 (68%) achieved successful live delivery. In the extended trial, pregnancy was achieved in 156 (22.3% of 701) with 79 live deliveries (50.1%).

CONCLUSIONS:

The use of 830 nm LLLT in the proximal priority technique at the parameters used in the present study, on its own or as an adjunct to other techniques, resulted in successful induction of pregnancy in just over 21% of severely infertile females, with a substantial number of these achieving live births. No adverse events were noted in any patient. LLLT is a pain-free and sideeffect free modality which could give hope to the increasing numbers of older females with infertility in Japan and potentially worldwide. Multinational studies are warranted.

 

KEYWORDS:

Artificial reproductive technology (ART); In vitro fertilization (IVF); gamete intrafallopian transfer (GIFT); insemination by donor (AID); insemination by husband (AIH); proximal priority technique; severe infertility; zygote intrafallopian transfer (ZIFT)

Urologiia. 2003 Mar-Apr;(2):23-5.

[Etiopathogenetic basis for using magnetolaser therapy in the complex treatment of male infertility].

[Article in Russian]
Iurshin VV, Sergienko NF, Illarionov VE.

Abstract

Up to 12-15% couples are infertile. The "responsibility" for infertility rests with the husband in 40-45% cases. The effects of routine drug therapy (n = 95) on a generative function are compared to those of magnetolaser therapy (n = 93) in 188 males with excretory-inflammatory infertility suffering from chronic prostatitis. Low-intensity laser infra-red radiation was used in a permanent magnetic field produced by Azor-2K unit. The magnetolaser therapy more significantly than the routine therapy raised concentration and number of mobile forms of the spermia, reduced their degenerative forms, elevated the level of serum sexual and gonadotropic hormones. In 1 year pregnancy occurred in 41.7 and 55.4% of 83 and 87 families (groups 1 and 2), respectively. The delivery took place in 35.8 and 49.7%, respectively.

 

Vopr Kurortol Fizioter Lech Fiz Kult. 2009 Jan-Feb;(1):25-8.

Application of low-intensity laser radiation and endotoxin-binding preparations to the treatment of female infertility

[Article in Russian]

Enukidze GG.

A total of 38 women of reproductive age (from 20 to 45 years) with chronic inflammatory gynecological diseases including 7 with primary and 9 with secondary infertility were examined by standard clinical, instrumental, and laboratory methods. In addition, variations of such important characteristics as serum endotoxin level and activity of antiendotoxin immunity were measured. The study has demonstrated participation of chronic aggression of endotoxins (of intestinal origin) in pathogenesis of the disorders of interest. Inclusion of the "antiendotoxic component" in the combined therapy allowed the efficacy of the treatment of chronic inflammation and female infertility to be greatly enhanced. It suggests the important (if not decisive) role of bacterial lipopolysacchardides in the pathogenetic mechanism underlying the problems considered in this study.

Urologiia. 2003 Mar-Apr;(2):23-5.  

Etiopathogenetic basis for using magnetolaser therapy in the complex treatment of male infertility

[Article in Russian]

Iurshin VV, Sergienko NF, Illarionov VE.

Up to 12-15% couples are infertile. The "responsibility" for infertility rests with the husband in 40-45% cases. The effects of routine drug therapy (n = 95) on a generative function are compared to those of magnetolaser therapy (n = 93) in 188 males with excretory-inflammatory infertility suffering from chronic prostatitis. Low-intensity laser infra-red radiation was used in a permanent magnetic field produced by Azor-2K unit. The magnetolaser therapy more significantly than the routine therapy raised concentration and number of mobile forms of the spermia, reduced their degenerative forms, elevated the level of serum sexual and gonadotropic hormones. In 1 year pregnancy occurred in 41.7 and 55.4% of 83 and 87 families (groups 1 and 2), respectively. The delivery took place in 35.8 and 49.7%, respectively.

Vopr Kurortol Fizioter Lech Fiz Kult. 1994 Mar-Apr;(2):24-6.  

The use of laser therapy for restoring the fertilizing capacity of the ejaculate in men with a chronic genital inflammation

[Article in Russian]

Voronin IuT.

The study aimed at investigation of laser radiation effect on reproductive male function which has failed as a result of genital inflammation, versus the efficacy of routine chemotherapy. The treatment was given to 50 males of reproductive age who had been infertile for 1-12 years. 25 of them (group 1) were exposed to laser, the other 25 received standard drugs. The responses were assessed clinically and by ejaculate potency. Due to laser application clinical and ejaculate characteristics improved in the absence of side effects either on the reproductive system or the body as a whole. The author recommends laser application for treatment of ejaculate infertility in males with chronic genital inflammation.

The transforming role of biological acceptor in the reaction of a low-intensive laser irradiation.

Burlakov A B et al.

The influence of low level laser on unfertilized oocytes and spermatozoons of fish was studied. HeNe and GaAs 862 nm was used. High quality eggs (fertilization above 70%) were not influenced by laser light. The development in eggs of mean quality (fertilization 30-60%) was boosted and the best effect was found in poor quality eggs (below 20%). The fertilization rate and the reduction of the number of abnormal developing embryos was measured. After temperatural inactivation both oocytes and spermatozoons, the irradiation not only restored the movability and fertilizating capacity, but also promoted the development of inactivated oocytes after fertilization by the irradiated sparmatozoons. Red and infrared light had different effects.

Lasers Med Sci. 2005 Apr 19; [Epub ahead of print

Effect of 655-nm diode laser on dog sperm motility

Corral-Baques MI, Rigau T, Rivera M, Rodriguez JE, Rigau J.

Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Rovira i Virgili University, Reus, Spain.

Sperm motility depends on energy consumption. Low-level laser irradiation increases adenosin triphosphate (ATP) production and energy supply to the cell. The aim of this study is to analyse whether the irradiation affects the parameters that characterise dog sperm motility. Fresh dog sperm samples were divided into four groups and irradiated with a 655-nm continuous-wave diode laser with varying doses: 0 (control), 4, 6 and 10 J/cm(2). At 0, 15 and 45 min following irradiation, pictures were taken of all the groups in order to study motility with computer-aided sperm analysis (CASA). Functional tests were also performed. Average path velocity (VAP), linear coefficient (Lin) and beat cross frequency (BCF) were statistically and significantly different when compared to the control. The functional tests also showed a significant difference. At these parameters, the 655-nm continuous-wave diode laser improves the speed and linear coefficient of the sperm.

Can HeNe laser improve fertility?

Abstracts LASERmed 97;  p. 138, no 112

The fertilizing potential of mouse spermatozoa was positively affected by HeNe laser in vitro. Cohen et al at the Bar-Ilan University, Israel found that the Ca2+ uptake, mainly in the mitochondria, was improved after LLLT. The results suggest that the effect of 630 nm laser irradiation is mediated through the generation of hydrogren peroxide by the spermatozoa and that this effect plays an important role in the augmentation of the sperm cell’s capacity to fertilize in vitro.